gel filtration column Search Results


90
Tosoh Corporation gel filtration chromatography analysis
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Gel Filtration Chromatography Analysis, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Life Sciences Inc gel filtration column s200gl 10/300
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Gel Filtration Column S200gl 10/300, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Life Sciences Inc gel filtration column aca44
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Gel Filtration Column Aca44, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Pharmacia Biotech Ltd sephacryl-s300rh gel filtration column chromatography
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Sephacryl S300rh Gel Filtration Column Chromatography, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Pharmacia Biotech Ltd gel filtration chromatography (g-25 column
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Gel Filtration Chromatography (G 25 Column, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Salipro Biotech AB superdex s200 analytical gel filtration column
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Superdex S200 Analytical Gel Filtration Column, supplied by Salipro Biotech AB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Tosoh Corporation tsk-gel4000×1
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Tsk Gel4000×1, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Life Sciences Inc gel filtration 16/60 superdex column
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Gel Filtration 16/60 Superdex Column, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Tosoh Corporation up sw 3000 gel filtration column
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Up Sw 3000 Gel Filtration Column, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Pharmacia Biotech Ltd 16×600 mm superdex 75 gel filtration column
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
16×600 Mm Superdex 75 Gel Filtration Column, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Cold Spring Harbor Laboratory Meetings gel permeation chromatography
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Gel Permeation Chromatography, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen dyeex 2.0 gel filtration columns
Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel <t>filtration</t> <t>chromatography</t> (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Dyeex 2.0 Gel Filtration Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel filtration chromatography (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.

Journal: Frontiers in Plant Science

Article Title: Kinetic analysis of the interactions between plant thioredoxin and target proteins

doi: 10.3389/fpls.2013.00508

Figure Lengend Snippet: Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel filtration chromatography (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.

Article Snippet: Gel filtration chromatography analysis was performed using a TSK-G2000SW XL (Tosoh, Tokyo), which had been equilibrated with H buffer.

Techniques: Injection, Filtration, Chromatography, Column Chromatography, Incubation